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Which of the following single base substitutions in the transcribed strand of DNA would result in premature termination of translation?


A) CAA to CAG
B) CCT to CCC
C) ATG to ATT
D) GAG to TAG
E) CTG to CTT

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The binding of snRNPs to consensus sequences is necessary for


A) gene duplication.
B) the addition of a poly A tail.
C) capping an hnRNA.
D) RNA splicing.
E) transcription.

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Which of the following statements about RNA polymerase is false?


A) It synthesizes mRNA in a 5´-to-3´ direction, reading the DNA strand 3´ to 5´.
B) It synthesizes mRNA in a 3´-to-5´ direction, reading the DNA strand 5´ to 3´.
C) It binds at the promoter and unwinds the DNA.
D) It does not require a primer to initiate transcription.
E) It uses only one strand of DNA as a template for synthesizing RNA.

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The genetic code


A) is different for prokaryotes and eukaryotes.
B) has changed during the course of recent evolution.
C) has 64 codons that code for amino acids.
D) has more than one codon for many amino acids
E) is ambiguous.

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The fact that some tRNA molecules do not have to pair exactly is called _______.

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Proteins are synthesized from the _______, in the _______ direction along the mRNA.


A) N terminus to C terminus; 5´ to 3´
B) C terminus to N terminus; 5´ to 3´
C) C terminus to N terminus; 3´ to 5´
D) N terminus to C terminus; 3´ to 5´
E) N terminus to N terminus; 5´ to 5´

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Beadle and Tatum proposed the one-gene, one-enzyme hypothesis. Why did this hypothesis have to be modified?


A) Some RNAs have catalytic activity.
B) Not all proteins are enzymes.
C) Some enzymes are made up of more than one polypeptide.
D) Not all genes code for enzymes.
E) Not all enzymes are coded for by genes.

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Which of the following is not part of RNA processing in eukaryotes?


A) Splicing of exons
B) Reverse transcription
C) Addition of a 5´ cap
D) Addition of a poly A tail
E) Intron removal

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Translation of messenger RNA into protein occurs in a _______ direction, and from _______ terminus to _______ terminus.


A) 3´-to-5´; N; C
B) 5´-to-3´; N; C
C) 3´-to-5´; C; N
D) 5´-to-3´; C; N
E) 3´-to-5´; C; C

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Order the four steps in eukaryotic gene expression given below from beginning to end. (1) Pre-mRNA is processed to make mRNA. (2) Ribosomes translate the mRNA message to make proteins. (3) mRNA is transported to the cytoplasm. (4) DNA is used as a template make pre-mRNA.


A) 1; 4; 3; 2
B) 4; 3; 1; 2
C) 4; 1; 3; 2
D) 4; 1; 2; 3
E) 1; 2; 4; 3

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Ribosomes are a collection of _______ that are needed for _______.


A) small proteins; translation
B) proteins and small RNAs; translation
C) proteins and tRNAs; transcription
D) proteins and mRNAs; translation
E) mRNAs and tRNAs; translation

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Termination codons


A) are not part of the genetic code.
B) bind tRNAs.
C) bind mRNA factor.
D) stop translation.
E) stop transcription.

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Peptidyl transferase is an


A) enzyme found in the nucleus of the cell that assists in the transfer of mRNA to the cytoplasm.
B) enzyme that adds the amino acid to the 3´ end of the tRNA.
C) enzyme found in the large subunit of the ribosome that catalyzes the formation of the peptide bond in the growing polypeptide.
D) RNA molecule that is catalytic.
E) Both c and d

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RNA polymerase is


A) an RNA-directed DNA polymerase.
B) an RNA-directed RNA polymerase.
C) a DNA-directed RNA polymerase.
D) a typical enzyme.
E) a form of RNA.

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Exons are


A) spliced out of the original transcript.
B) spliced together from the original transcript.
C) spliced to introns to form the final transcript.
D) much larger than introns.
E) larger than the original coding region.

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Which of the following is not a difference between gene expression in prokaryotes and eukaryotes?


A) There are several different RNA polymerases in both prokaryotes and eukaryotes.
B) In eukaryotes, RNA polymerase requires transcription factors or initiation.
C) Prokaryotic genes are often grouped together.
D) In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
E) There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

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An mRNA has the sequence 5´-AUGAAAUCCUAG-3´. What is the template DNA strand for this sequence?


A) 5´-TACTTTAGGATC-3´
B) 5´-ATGAAATCCTAG-3´
C) 5´-GATCCTAAAGTA-3´
D) 5´-TACAAATCCTAG-3´
E) 5´-CTAGGATTTCAT-3´

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How many codons specify amino acids?


A) 20
B) 23
C) 45
D) 60
E) 61

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If the following synthetic RNA were added to a test tube containing all the components necessary for protein translation to occur, what would the amino acid sequence be? 5´ - A U A U A U A U A U A U - 3´


A) Polyphenylalanine
B) Isoleucine-tyrosine-isoleucine-tyrosine
C) Isoleucine-isoleucine-isoleucine-isoleucine
D) Tyrosine-tyrosine-tyrosine-tyrosine
E) Aspargine-aspargine-aspargine-aspargine

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Prototrophs ("original eaters") grow on minimal media, whereas _______ ("increased eaters") require specific additional nutrients.

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