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In order to show that DNA in cell extracts is responsible for genetic transformation in Streptococcus pneumoniae, important corroborating evidence should indicate that _______ also destroy transforming activity. a. enzymes that hydrolyze proteins b. enzymes that hydrolyze DNA c. enzymes that hydrolyze polysaccharides d. temperatures high enough to kill cells e. enzymes that hydrolyze RNA

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Which DNA repair system or mechanism acts as the second line of defense against mutational error that occurs as the result of DNA replication?


A) Excision
B) Mismatch
C) Proofreading
D) Telomerase
E) Ligation

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In DNA replication, on the _______, the exposed DNA 3 end gets farther and farther away from the fork as replication proceeds.


A) leading strand
B) lagging strand
C) Okazaki fragments
D) primer
E) sliding DNA clamp

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Mammalian _______ is the eukaryotic equivalent of bacterial transformation.

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Four rounds of PCR will result in _______ molecules of DNA from a single molecule.

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Suppose proteins, and not DNA, were the genetic material in bacteriophage T2.What would we expect to find in the host cells in the Hershey and Chase blender experiment?


A) Most of the labeled S
B) Most of the labeled P
C) Both labeled S and labeled P, with S more prevalent than P
D) Both labeled S and labeled P, with P more prevalent than S
E) Neither labeled S nor labeled P

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Which statement about Okazaki fragments is true?


A) They occur because DNA polymerase operates in only one direction along a strand of DNA.
B) They act as a primer that initiates DNA replication.
C) If they did not exist, the ends of chromosomes would get shorter with every replication.
D) It they did not exist, bases would pair with their complementary bases.
E) They reduce the mutation rate during DNA replication.

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Six complete cycles of PCR should result in a _______-fold increase in the amount of DNA. a. 6 b. 12 c. 32 d. 64 e. 128

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What enables the specificity of PCR?


A) A heat-tolerant DNA polymerase
B) The use of particular buffers
C) The use of particular primers
D) The ratios of dNTPs used
E) The high temperature used to denature DNA

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Recall that two hydrogen bonds bind A and T, but three hydrogen bonds bind G and C.Since the stability of DNA is affected by the number of hydrogen bonds, with more bonds promoting more stability, which of the following double-stranded DNA molecules would likely have the greatest stability?


A) A molecule with 20 percent A
B) A molecule with 26 percent G
C) A molecule with 32 percent C
D) A molecule with 38 percent T
E) More information is required to answer the question.

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The Hershey and Chase blender experiment was designed to


A) prove that DNA is found in the nucleus.
B) test isotope labeling of DNA and proteins.
C) disprove that DNA is the genetic molecule.
D) identify the genetic material in bacteriophage T2.
E) develop a DNA isolation method.

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Refer to the figure. Refer to the figure.   Which structure illustrates a replication fork? A)  A B)  B C)  C D)  D E)  E Which structure illustrates a replication fork?


A) A
B) B
C) C
D) D
E) E

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The MLH1 protein dimerizes with the PMS2 protein to form a complex that is involved in the correction of errors of DNA replication.Loss-of-function mutations in the genes that encode for these proteins increase the incidence of colon cancer.Which type of DNA error would be repaired by the activity of this complex?


A) Mismatched bases
B) Chemically induced damage to nucleotides
C) Thymine dimers
D) Radiation-induced damage to nucleotides
E) All types of DNA errors

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Within a double-stranded DNA molecule, an A on one strand is paired with a G on the other.Which DNA repair system would most likely fix this error?


A) Proofreading
B) Excision
C) Ligase
D) Mismatch
E) Template

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Which type of DNA repair system can repair errors in single-stranded DNA?


A) Excision
B) Mismatch
C) Proofreading
D) Polymerase
E) None of the above

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A researcher would like to radioactively label DNA for an experiment.Which type of molecule could not serve as a target for radioactive labeling?


A) Purines
B) Ribose sugars
C) Phosphates
D) Sulfur
E) Nitrogenous bases

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In PCR, the temperature is lowest when


A) DNA is separated into two strands.
B) primers anneal with the template.
C) DNA polymerase is producing new strands of DNA.
D) DNA is being repaired.
E) the temperature is the same during all of the stages of PCR.

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Explain the ways that DNA polymerase participates in maintaining the fidelity of the DNA.

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DNA polymerase participates in...

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Which statement about telomerase is true?


A) It prevents the ends of chromosomes from continuing to grow.
B) It prevents the ends of chromosomes from being eroded with each round of DNA replication.
C) It makes DNA repair possible.
D) It is necessary for the formation of Okazaki fragments.
E) It is necessary for the formation of hydrogen bonds between complementary bases.

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The enzyme that restores the phosphodiester linkage between adjacent fragments in the lagging strand during DNA replication is


A) DNA ligase.
B) primase.
C) reverse transcriptase.
D) helicase.
E) DNA polymerase I.

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