Question 1

Eukaryotic and bacterial RNA polymerases are different such that:

Accepted Answer

A)  eukaryotes have several types of RNA polymerases,whereas bacteria only have one.
B)  unlike bacterial RNA polymerase,eukaryotic RNA pol II can synthesize mRNA and also coordinate mRNA processing.
C)  eukaryotic RNA polymerases are multimeric,whereas bacterial RNA polymerase is not.
D)  eukaryotes have several types of RNA polymerases,one of which can synthesize mRNA and also coordinate mRNA processing,whereas bacteria only have one type of RNA polymerase that does not coordinate mRNA processing.
E)  eukaryotes have several types of multimeric RNA polymerases,whereas bacteria only have one monomeric RNA polymerase.

Question 2

In the bacterial lac operon,the physiologically regulated step is:

Accepted Answer

A)  DNA-binding by the transcriptional regulator.
B)  activity of the activation-domain of the transcriptional regulator.
C)  feedback inhibition by the end-product of the pathway.
D)  import of the inducer molecule.
E)  None of the answer options are correct.

Question 3

Describe the difference between transcriptional gene regulation and post-transcriptional gene regulation in eukaryotes.

Accepted Answer

In transcriptional gene regulation,regul...

Question 4

Histones are essentially identical in sequence/structure in all eukaryotic organisms from yeast to plants to animals.What does this say about the biophysical properties of DNA-packaging and the evolution of eukaryotic organisms?

Accepted Answer

Histones are basic proteins able to inte...

Question 5

The phenomenon in D.melanogaster known as Position-Effect Variegation (PEV) provides powerful evidence that:

Accepted Answer

A)  DNA is the genetic material.
B)  posttranscriptional mechanisms regulate gene expression.
C)  transcriptional mechanisms regulate gene control.
D)  chromatin structure is unable to regulate gene expression.
E)  chromatin structure is able to regulate gene expression.

Question 6

Epigenetic inheritance is defined as the inheritance of:

Accepted Answer

A)  nuclear DNA from one cell generation to the next.
B)  extra-chromosomal DNA from one cell generation to the next.
C)  mRNA from one cell generation to the next.
D)  chromatin states from one cell generation to the next.
E)  None of the answer options are correct.

Question 7

In the eukaryotic GAL system,the physiologically regulated step is

Accepted Answer

A)  DNA-binding by the transcriptional regulator.
B)  activity of the activation-domain of the transcriptional regulator.
C)  feedback inhibition by the end-product of the pathway.
D)  import of the inducer molecule.
E)  None of the answer options are correct.

Question 8

The "domain-swapping" experiment that grafts the Gal4 activation domain to the LexA DNA-binding domain generates a chimeric protein that will:

Accepted Answer

A)  bind to the Gal4 site.
B)  bind to the LexA site.
C)  activate transcription of the Gal4 gene.
D)  activate transcription of the Gal7 gene.
E)  All of the answer options are correct.

Question 9

Regions of a chromosome that are packaged in less-condensed chromatin are known as:

Accepted Answer

A)  homochromatin.
B)  heterochromatin.
C)  euchromatin.
D)  epichromatin.
E)  hyperchromatin.

Question 10

The ________________ is a cis-acting element that binds RNA polymerase II.

Accepted Answer

A)  promoter
B)  enhancer
C)  promoter-proximal element
D)  GC-rich box
E)  All of the answer options are correct.

Question 11

What portion of a histone is typically modified by acetylation/de-acetylation?

Accepted Answer

A)  acidic amino acid residues in the "head" region of the histone
B)  basic amino acid residues in the "head" region of the histone
C)  basic amino acid residues in the "tail" region of the histone
D)  acidic amino acid residues in the "head" region and basic amino acid residues in the "tail" region
E)  None of the answer options are correct.

Question 12

In eukaryotes,transcriptional gene control is mediated by:

Accepted Answer

A)  trans-acting factors binding to cis-acting elements.
B)  trans-acting factors failing to bind to cis-acting elements.
C)  repressor proteins binding to operator sites.
D)  metabolites binding to cis-acting elements.
E)  None of the answer options are correct.

Question 13

What is chromatin remodeling?

Accepted Answer

A)  the first stage of mitosis
B)  the changing of amino acid position
C)  the changing of nucleosome position
D)  the changing of nucleotide position
E)  None of the answer options are correct.

Question 14

In mice,the igf2 allele is only expressed if it is:

Accepted Answer

A)  inherited from the mother.
B)  inherited from the father.
C)  mutated in the mother.
D)  mutated in the father.
E)  None of the answer options are correct.

Question 15

Analyzing mutations in the GAL80 and GAL3 indicate that:

Accepted Answer

A)  both GAL80 and GAL3 inhibit GAL gene expression.
B)  both GAL80 and GAL3 promote GAL gene expression.
C)  GAL80 promotes GAL gene expression,and GAL3 inhibits GAL gene expression.
D)  GAL80 inhibits GAL gene expression,and GAL3 promotes GAL gene expression.
E)  GAL80 and GAL3 are not involved in GAL expression.

Question 16

A Barr body refers to:

Accepted Answer

A)  a sex-linked trait in cats.
B)  a recessive trait in fruit flies.
C)  an inactivated Y chromosome.
D)  spore formation in Neurospora.
E)  None of the answer options are correct.

Question 17

What is an enhanceosome?

Accepted Answer

A)  a large complex that acts synergistically to activate translation
B)  a large complex that acts synergistically to activate transcription
C)  an aggregation of nucleosomes that activates transcription
D)  an aggregation of nucleosomes that activates translation
E)  a large complex that promotes epigenetic inheritance

Question 18

The enzyme responsible for adding acetyl groups to histone proteins is called:

Accepted Answer

A)  histone acetylase (H
A)  .
B)  histone deacetylase (HDA
C)  .
C)  histone acetylmethyltransferase (HAT) .
D)  histone acetyltransferase (HAT) .
E)  histone methyltransferase (HMT) .

Question 19

Which of the following describes the histones associated with the nucleosomes of active genes?

Accepted Answer

A)  poor in acetyl groups (i.e. ,hypoacetylated) 
B)  rich in acetyl groups (i.e. ,hyperacetylated) 
C)  rich in phosphate groups (i.e. ,hyperphosphorylated) 
D)  poor in phosphate groups (i.e. ,hypophosphorylated) 
E)  None of the answer options are correct.

Question 20

In mice,the H19 allele is only expressed if it is:

Accepted Answer

A)  inherited from the mother.
B)  inherited from the father.
C)  mutated in the mother.
D)  mutated in the father.
E)  None of the answer options are correct.

Exam 12: Regulation of Gene Expression in Eukaryotes

The enzyme responsible for adding acetyl groups to histone proteins is called:

Correct AnswerverifiedShow Answer

The ________________ is a cis-acting element that binds RNA polymerase II.

Correct AnswerverifiedShow Answer

In the bacterial lac operon,the physiologically regulated step is:

Correct AnswerverifiedShow Answer

In mice,the H19 allele is only expressed if it is:

Correct AnswerverifiedShow Answer

Describe the difference between transcriptional gene regulation and post-transcriptional gene regulation in eukaryotes.

Correct AnswerverifiedIn transcriptional gene regulation,regul...View AnswerShow Answer

What portion of a histone is typically modified by acetylation/de-acetylation?

Correct AnswerverifiedShow Answer

What is an enhanceosome?

Correct AnswerverifiedShow Answer

Which of the following describes the histones associated with the nucleosomes of active genes?

Correct AnswerverifiedShow Answer

The phenomenon in D.melanogaster known as Position-Effect Variegation (PEV) provides powerful evidence that:

Correct AnswerverifiedShow Answer

Analyzing mutations in the GAL80 and GAL3 indicate that:

Correct AnswerverifiedShow Answer

What is chromatin remodeling?

Correct AnswerverifiedShow Answer

In eukaryotes,transcriptional gene control is mediated by:

Correct AnswerverifiedShow Answer

In the eukaryotic GAL system,the physiologically regulated step is

Correct AnswerverifiedShow Answer

The "domain-swapping" experiment that grafts the Gal4 activation domain to the LexA DNA-binding domain generates a chimeric protein that will:

Correct AnswerverifiedShow Answer

In mice,the igf2 allele is only expressed if it is:

Correct AnswerverifiedShow Answer

Regions of a chromosome that are packaged in less-condensed chromatin are known as:

Correct AnswerverifiedShow Answer

Histones are essentially identical in sequence/structure in all eukaryotic organisms from yeast to plants to animals.What does this say about the biophysical properties of DNA-packaging and the evolution of eukaryotic organisms?

Correct AnswerverifiedHistones are basic proteins able to inte...View AnswerShow Answer

A Barr body refers to:

Correct AnswerverifiedShow Answer

Eukaryotic and bacterial RNA polymerases are different such that:

Correct AnswerverifiedShow Answer

Epigenetic inheritance is defined as the inheritance of:

Correct AnswerverifiedShow Answer

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In transcriptional gene regulation,regul...
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Histones are basic proteins able to inte...
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Correct Answer
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