Question 1

Which of the following is required for the lambda phage to cause lysis in a bacterial cell?

Accepted Answer

A)   Precise excision of phage DNA from the host chromosome
B)   Lambda integrase
C)   Lambda excisase
D)   All of these
E)   None of these

Question 2

Which of the following is a true statement regarding the bacteriophage lambda? 1.It is smaller than T4
2) It can undergo a lytic or lysogenic lifecycle
3) It is comprised of a double stranded linear piece of DNA encased in a protein coat

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 3
E)   All of these

Question 3

In a lysogenic pathway,the viral DNA is: 1.Inserted into the host chromosome
2) Replicated with the host chromosome
3) Integrated by site specific recombination

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 2
E)   All of these E

Question 4

Which of the following statements is false with regards to bacteria as a model organism for genetic study?

Accepted Answer

A)   Genetic variability is difficult to detect.
B)   Bacteria are ideal for studying fundamental biological processes because they are simple in structure.
C)   Bacteria can grow on artificial media that can be altered by the researcher.
D)   Bacteria can reproduce more quickly than Drosophila
E)   All of these are false A

Question 5

Which of the following is a true statement about the phage T4?
1) It is a temperate phage
2) It is a virulent phage
3) It can infect the bacterium E.coli

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 3
E)   2 and 3

Question 6

Which of the following statements is true with regard to viruses? 1.Viruses can only reproduce by infecting living cells
2) Viruses can be considered both living and non-living depending on the state they are in
3) Viruses that infect bacterial cells are known as bacteriophages

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 2
E)   All of these

Question 7

Temperate and virulent phages differ in that:

Accepted Answer

A)   Virulent phages kill the host cell and temperate phages never kill the host cell
B)   Virulent phages never kill the host cell and temperate phages always kill the host cell
C)   Virulent phages kill the host cell and temperate phages can kill the host cell or live in a symbiotic state with the host cell
D)   Temperate phages kill the host cell and virulent phages can kill the host cell or live in a symbiotic state with the host cell
E)   All of these are true

Question 8

When bacteriophage l integrates into the bacterial chromosome,it does so at:

Accepted Answer

A)   An att P site in the host chromosome.
B)   An att B site in the phage chromosome.
C)   An att B site in the host chromosome using a lambda-encoded integrase
D)   An att B site in the host chromosome using a bacteria-encoded integrase.
E)   An att B site in both the host and phage chromosomes

Question 9

Which of the following is true regarding bacteria?

Accepted Answer

A)   Bacteria reproduce by simple fission
B)   Bacteria are monoploid and multinucleate
C)   Bacteria do not undergo mitosis
D)   Bacteria do not undergo meiosis
E)   All of these are true

Question 10

Plaque morphology is a function of:

Accepted Answer

A)   A physiological interaction between bacteria and phage.
B)   Phage genotype
C)   Bacterial strain
D)   All of these
E)   None of these

Question 11

Which of the following can be mutated in bacteria? 1.Colony morphology
2) Cell morphology
3) Ability to use an energy source

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 2
E)   All of these

Question 12

The genetic map of phage T4 is circular because: 1.The sequence is terminally redundant
2) The sequence is circularly permuted
3) The sequence is 50kbp long

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 2
E)   2 and 3

Question 13

The lambda phage can cause lysis in a bacterium when: 1.The lamda DNA is spontaneously excised from the host chromosome
2) The lambda DNA is induced to excise from the host chromosome
3) The lambda DNA excises from the host chromosome and moves back into the phage

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 2
E)   All of these

Question 14

The modified nitrogenous base found in the T4 phage DNA also contains an attached glucose derived residue that is used for what purpose?

Accepted Answer

A)   To increase transcription from phage promoters
B)   To protect phage from phage encoded nucleases
C)   To protect phage from host encoded restriction enzymes.
D)   To indicate a site for head-full packaging
E)   All f these

Question 15

Which of the following is a true statement about the phage Lambda? 1.It is a temperate phage
2) It is a virulent phage
3) It can infect the bacterium E.coli

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 3
E)   2 and 3

Question 16

In its integrated state the lambda chromosome is known as a:

Accepted Answer

A)   Lyophilized phage
B)   Prophage
C)   Metaphage
D)   Lytic phage
E)   None of these

Question 17

Plasmids are: 1.Autonomously replicating
2) Circular
3) Extrachromosomal DNA

Accepted Answer

A)   1
B)   2
C)   3
D)   1 and 3
E)   All of these E

Question 18

Two of the most important bacteriophages that have played a role in they study of genetics are:

Accepted Answer

A)   E. coli and T4
B)   T4 and TMZ
C)   T4 and Lambda
D)   Lambda and E. coli
E)   Lambda and TMZ

Question 19

The biological purpose of T4 lysozyme during phage propagation is to:

Accepted Answer

A)   Open the phage head to release viral DNA into the host cell.
B)   Disrupt the nuclear membrane of the host cell.
C)   Lyse host bacteria to release newly formed phage.
D)   Assemble the head and tail fibers of new phages.
E)   Shut off transcription and translation of bacterial genes in the cell.

Question 20

The head-full mechanism of DNA packaging incorporates _____________ of T4 DNA per phage head.

Accepted Answer

A)   more than one genome
B)   less than one genome
C)   one genome
D)   all of these
E)   none of these

Exam 8: The Genetics Of Bacteria And Their Viruses

Which of the following is required for the lambda phage to cause lysis in a bacterial cell?

Correct AnswerverifiedShow Answer

Which of the following is a true statement regarding the bacteriophage lambda? 1.It is smaller than T4 2) It can undergo a lytic or lysogenic lifecycle 3) It is comprised of a double stranded linear piece of DNA encased in a protein coat

Correct AnswerverifiedShow Answer

In a lysogenic pathway,the viral DNA is: 1.Inserted into the host chromosome 2) Replicated with the host chromosome 3) Integrated by site specific recombination

Correct AnswerverifiedE

Which of the following statements is false with regards to bacteria as a model organism for genetic study?

Correct AnswerverifiedA

Which of the following is a true statement about the phage T4? 1) It is a temperate phage 2) It is a virulent phage 3) It can infect the bacterium E.coli

Correct AnswerverifiedShow Answer

Which of the following statements is true with regard to viruses? 1.Viruses can only reproduce by infecting living cells 2) Viruses can be considered both living and non-living depending on the state they are in 3) Viruses that infect bacterial cells are known as bacteriophages

Correct AnswerverifiedShow Answer

Temperate and virulent phages differ in that:

Correct AnswerverifiedShow Answer

When bacteriophage l integrates into the bacterial chromosome,it does so at:

Correct AnswerverifiedShow Answer

Which of the following is true regarding bacteria?

Correct AnswerverifiedShow Answer

Plaque morphology is a function of:

Correct AnswerverifiedShow Answer

Which of the following can be mutated in bacteria? 1.Colony morphology 2) Cell morphology 3) Ability to use an energy source

Correct AnswerverifiedShow Answer

The genetic map of phage T4 is circular because: 1.The sequence is terminally redundant 2) The sequence is circularly permuted 3) The sequence is 50kbp long

Correct AnswerverifiedShow Answer

The lambda phage can cause lysis in a bacterium when: 1.The lamda DNA is spontaneously excised from the host chromosome 2) The lambda DNA is induced to excise from the host chromosome 3) The lambda DNA excises from the host chromosome and moves back into the phage

Correct AnswerverifiedShow Answer

The modified nitrogenous base found in the T4 phage DNA also contains an attached glucose derived residue that is used for what purpose?

Correct AnswerverifiedShow Answer

Which of the following is a true statement about the phage Lambda? 1.It is a temperate phage 2) It is a virulent phage 3) It can infect the bacterium E.coli

Correct AnswerverifiedShow Answer

In its integrated state the lambda chromosome is known as a:

Correct AnswerverifiedShow Answer

Plasmids are: 1.Autonomously replicating 2) Circular 3) Extrachromosomal DNA

Correct AnswerverifiedE

Two of the most important bacteriophages that have played a role in they study of genetics are:

Correct AnswerverifiedShow Answer

The biological purpose of T4 lysozyme during phage propagation is to:

Correct AnswerverifiedShow Answer

The head-full mechanism of DNA packaging incorporates _____________ of T4 DNA per phage head.

Correct AnswerverifiedShow Answer

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