Question 1

The annealing step in a PCR involves:

Accepted Answer

A)   binding of the primers to the single-stranded DNA.
B)   unwinding and separating the double-stranded DNA.
C)   adding nucleotides to primed sites of the DNA strands.
D)   making a cDNA from an mRNA strand.

Question 2

You have prepared a restriction enzyme digest of DNA from an individual who might have a genetic disorder.On the gel,you note that the digest from this individual produces one fragment,whereas the digest from a normal healthy individual produces two fragments.All controls worked correctly and you used the same reagents for all testing.What is your interpretation?

Accepted Answer

A)   The enzymes were not working.
B)   The diseased individual's DNA is missing a restriction site.
C)   You must have skipped the amplification step.
D)   The normal individual's DNA is missing a restriction site.

Question 3

When combined with real-time PCR,melting curve analysis is done to:

Accepted Answer

A)   examine a single nucleotide variant genotype in a short amount of time.
B)   quickly quantify the amplified target.
C)   detect nucleic molecules at a specific wavelength.
D)   examine the size of a fragment produced by RFLP.

Question 4

When performing a PCR procedure,what is the best control to run to assess the presence of amplicon?

Accepted Answer

A)   Positive control
B)   Blank control
C)   Oligoligated control
D)   dTTP control

Question 5

In a PCR,a control nucleic acid sequence that is different from the target sequence is added to each sample to:

Accepted Answer

A)   control for the presence of amplicon contamination.
B)   bind aerosols.
C)   control for the presence of inhibitors in the sample that might inhibit polymerase activity.
D)   compare with an internal standard for quantitative analysis.

Exam 48: Nucleic Acid Techniques and Applications

When performing a PCR procedure,what is the best control to run to assess the presence of amplicon?

Correct Answer
verified

In a PCR,a control nucleic acid sequence that is different from the target sequence is added to each sample to:

Correct Answer
verified

The annealing step in a PCR involves:

Correct Answer
verified

When combined with real-time PCR,melting curve analysis is done to:

Correct Answer
verified

Correct Answer
verified

Exam 48: Nucleic Acid Techniques and Applications

When performing a PCR procedure,what is the best control to run to assess the presence of amplicon?

Correct AnswerverifiedShow Answer

In a PCR,a control nucleic acid sequence that is different from the target sequence is added to each sample to:

Correct AnswerverifiedShow Answer

The annealing step in a PCR involves:

Correct AnswerverifiedShow Answer

When combined with real-time PCR,melting curve analysis is done to:

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified