Question 1

The primers in PCR are

Accepted Answer

A)   synthetic DNA oligonucleotides.
B)   reverse transcriptases.
C)   bacterial enzymes.
D)   DNA polymerases.
E)   short RNA strands.

Question 2

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA technology
D)   gel electrophoresis
E)   gene probe technology

Question 3

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Accepted Answer

A) True 
 B)False

Question 4

Analysis of DNA fragments in gel electrophoresis is based on

Accepted Answer

A)   larger fragments moving slowly and remaining closer to the wells.
B)   DNA having an overall negative charge and moving to the positive pole.
C)   DNA fragments being stained so that they can be seen.
D)   application of an electric current through the gel causing DNA fragments to migrate.
E)   All of the choices are correct.

Question 5

Common vectors used to transfer a piece of DNA into a cloning host are

Accepted Answer

A)   plasmids.
B)   viruses.
C)   bacteriophages.
D)   artificial chromosomes.
E)   All of the choices are correct.

Question 6

Why is an enzyme from a thermophilic bacterium used in PCR?

Accepted Answer

A)   The enzyme makes DNA that is more similar to human DNA.
B)   This thermohile's enzyme will synthesize DNA.
C)   DNA is replicated at a high temperature that denatures most proteins.
D)   It is cheaper to obtain from live microorganisms than producing the enzyme in a lab.

Question 7

The difference between somatic cell gene therapy and germline therapy is that

Accepted Answer

A)   germline therapy overcomes a protein malfunction in specific tissues but is not repaired in the entire organism and cannot be passed on to offspring.
B)   somatic cell gene therapy is introduced into the egg, sperm or developing embryo, whereas germline therapy introduces a new gene into a mature tissue.
C)   somatic cell gene therapy overcomes a protein malfunction in specific tissues but is not repaired in the entire organism and cannot be passed on to offspring.
D)   germline therapy is the temporary repair of a genetic mutation, whereas somatic cell therapy is a permanent fix.

Question 8

Gene probes can be labeled for detection with reporter molecules such as _____.

Accepted Answer

A)   enzymes
B)   fluorescent dyes
C)   radioisotopes
D)   All of the choices above can be used.

Question 9

Two different nucleic acids can _____ by annealing at their complementary sites.

Accepted Answer

A)   form a peptide bond
B)   covalently bond
C)   ligate
D)   hybridize

Question 10

What type of DNA map is most detailed?

Accepted Answer

A)   Linkage
B)   Sequence
C)   Physical
D)   Geographical
E)   Chromosomal

Question 11

Making new genomes is called ______.

Accepted Answer

A)   bioengineering
B)   synthetic biology
C)   genetic engineering
D)   cloning
E)   recombinant DNA

Question 12

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg,sperm,or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Accepted Answer

A) True 
 B)False

Question 13

The field of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Accepted Answer

A) True 
 B)False

Question 14

DNA strands can begin to separate at the temperature of ______.

Accepted Answer

A)   37oC
B)   42oC
C)   60oC
D)   90oC
E)   100oC

Question 15

Which PCR step synthesizes complimentary DNA strands?

Accepted Answer

A)  Heat target DNA to 94° C.
B)  Add DNA polymerase and nucleotides at 72° C.
C)  Repeat the cycle of heating and cooling.
D)  Cool DNA to between 50° C and 65° C.
E)  Add primers.

Question 16

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

A)   genes
B)   codons
C)   base pairs
D)   proteins
E)   triplets

Question 17

When patient tissues are transfected with viruses carrying a needed,normal human gene,the technique is called ______.

Accepted Answer

A)   cloning
B)   gene therapy
C)   antisense therapy
D)   DNA fingerprinting

Question 18

Which of the following is the second step in gene mapping?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host treated with calcium chloride and receives plasmid
C)   Separate DNA fragments with gel electrophoresis
D)   Desired protein is produced by cloning host
E)   Gene is amplified by multiplication of cloning host

Question 19

Amplification of DNA is accomplished by ______.

Accepted Answer

A)   a Southern blot
B)   a Western blot
C)   DNA sequencing
D)   gene probes
E)   the polymerase chain reaction

Question 20

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probes

Exam 10: Genetic Engineering and Recombinant Dna

It is possible to identify mRNA molecules using fluorescently labeled cDNA.

Correct AnswerverifiedShow Answer

DNA strands can begin to separate at the temperature of ______.

Correct AnswerverifiedShow Answer

Amplification of DNA is accomplished by ______.

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with reporter molecules such as _____.

Correct AnswerverifiedShow Answer

When patient tissues are transfected with viruses carrying a needed,normal human gene,the technique is called ______.

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complimentary DNA strands?

Correct AnswerverifiedShow Answer

The advantage of germline therapy over somatic cell therapy is that the normal gene is inserted into an egg,sperm,or developing embryo so that the repair is present in every cell of the organism as it matures to adulthood.

Correct AnswerverifiedShow Answer

Why is an enzyme from a thermophilic bacterium used in PCR?

Correct AnswerverifiedShow Answer

The various techniques by which scientists manipulate DNA in the lab are termed ______.

Correct AnswerverifiedShow Answer

The difference between somatic cell gene therapy and germline therapy is that

Correct AnswerverifiedShow Answer

Two different nucleic acids can _____ by annealing at their complementary sites.

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called ______.

Correct AnswerverifiedShow Answer

Common vectors used to transfer a piece of DNA into a cloning host are

Correct AnswerverifiedShow Answer

The field of pharmacogenomics uses knowledge of an individual's single nucleotide polymorphisms to determine how they will respond to a particular drug.

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis is based on

Correct AnswerverifiedShow Answer

Making new genomes is called ______.

Correct AnswerverifiedShow Answer

What type of DNA map is most detailed?

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedShow Answer

Which of the following is the second step in gene mapping?

Correct AnswerverifiedShow Answer

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