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Which of the following media will permit the growth of one group of bacteria while inhibiting the growth of some other groups?


A) Differential
B) Selective
C) Enrichment
D) Characteristic

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The Gram stain is used for bacteria that have cell walls while the acid-fast stain is used primarily for wall-less bacteria.

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Patients are instructed to collect sputum specimens in such a way as to minimize contamination with normal microbiota from the mouth.A sputum specimen has arrived in the clinical laboratory and a Gram-stained smear of the specimen reveals a mixture of Gram-positive cocci,Gram-negative cocci,and Gram-positive bacilli as well as numerous squamous epithelial cells.This result indicates


A) a poor quality specimen of primarily saliva and oropharyngeal microbiota.
B) that the specimen has been properly collected and is representative of sputum.
C) that the patient has a pneumonia caused by a mixture of different bacteria.
D) that the patient has bacterial pneumonia rather than viral pneumonia.

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A test which uses specific monoclonal antibodies to detect the presence of a pathogen (such as an indirect ELISA)is classified as a molecular genomic technique.

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Recommendations regarding hand-washing,handling of pipettes and sharps,prohibition of personal activities such as eating,applying makeup and handing contact lenses,directions regarding decontamination of work surfaces,cultures,and other potentially infectious material are all included in a set of guidelines known as ___________ ______________ __________________.

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standard microbiolog...

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The immunologic technique involving the transfer of proteins separated by electrophoresis to nitrocellulose sheets is _________.


A) complement fixation
B) direct immunofluorescence
C) immunoblotting
D) immunoelectrophoresis

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The purpose of the clinical microbiology laboratory is to maintain cultures of microorganisms.

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In the clinical laboratory,which of the following is (are) used as a rapid method of identification?


A) Manual biochemical kit systems
B) Mechanized/automated systems
C) Immunologic systems
D) All of the choices are correct.

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The most common method used by clinical microbiology labs for identifying bacteria are _________.


A) biochemical
B) morphological
C) PCR
D) DNA fingerprinting

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A colorless substrate acted on by the enzyme portion of the ligand to produce a colored product is known as a/an _________.


A) antibody
B) chromogen
C) antigen
D) serotype

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The immunological reaction that occurs when there is an optimal ratio of antigen and antibody,producing a lattice at the zone of equivalence is known _________.


A) immunoblotting
B) immunoelectrophoresis
C) immunodiffusion
D) immunoprecipitation

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Ten percent Calcofluor White stain is often used in wet mounts of _________.


A) bacteria
B) parasites
C) molds
D) protozoa

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Detection of single or multiple microorganisms based on their cytometric parameters is known as _________.


A) flow cytometry
B) complement fixation
C) agglutination
D) serotyping

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Standard microbiological practices include all of the following EXCEPT


A) work space should be disinfected only when contaminating spills occur.
B) eating,drinking,manipulation of contact lenses,and the use of cosmetics,gum,and tobacco products are strictly prohibited in the lab.
C) lab personnel should know how to use the emergency eyewash and shower stations.
D) hair longer than shoulder length should be tied back.

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An elevated IgM titer typically indicates an active infection while an elevated IgG titer does not distinguish between active and past infections.

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Which of the following uses red blood cells?


A) Hemagglutination inhibition
B) MIC
C) ELISA
D) RIA

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Which of the following can be examined with an ordinary bright-field microscope?


A) Wet mounts
B) Heat-fixed specimens
C) Chemical-fixed specimens
D) All of the choices are correct.

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Indirect immunofluorescence involves fixing the specimen onto a slide,adding fluorochrome-labeled antibodies and then incubating before examining on the microscope.

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An example of miniaturized commercial rapid bacterial identification system based on biochemical analysis is the


A) API 20E system.
B) Widal test.
C) Kirby-Bauer test.
D) All of the choices are correct.

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Clinical microbiologists diagnose some diseases by detecting and measuring the specific host response to the infection.What subdiscipline encompasses these methods?


A) Immunology
B) Microscopy
C) Physiology
D) Genetics
E) Ecology

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