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The clinical microbiology laboratory can provide preliminary identification of microorganisms based on


A) microscopic examination of specimens.
B) growth or biochemical characteristics.
C) immunologic techniques that detect microbial antigens.
D) All of the choices are correct.

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Fungal infections are often diagnosed by direct microscopic examination of specimens.

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Determining the susceptibility of a microorganism to specific chemotherapeutic agents is an important service performed in the clinical microbiology laboratory.

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An ELISA test to detect antibodies to the Rubella virus would include/use all of the following EXCEPT


A) microtiter wells coated with rubella antigen.
B) enzyme-labeled rabbit antihuman IgG antibodies.
C) a chromogenic enzyme substrate.
D) the patient serum specimen.
E) There are no exceptions here.Each of these items would be used.

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A patient specimen was Gram-stained upon arrival in the clinical laboratory and revealed both Gram-positive cocci and Gram-negative bacilli.The specimen was cultured on sheep blood agar and chocolate agar,and after incubation,the culture grew only the Gram-negative bacilli.Which of the following is/are a likely explanation(s) for the failure of the Gram-positive cocci to grow?


A) The Gram-negative bacilli may have grown very quickly and overgrew the slower growing Gram-positive cocci.
B) The media used may not support the growth of the Gram-positive cocci.
C) The growth temperature may have enabled the growth of the Gram-negative bacilli but not the growth of the Gram-positive cocci.
D) The Gram-positive cocci may be an obligate anaerobe and was unable to grow in an ambient air incubator.
E) All of the choices are possible explanations.

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E

Which of the following tests can be used to distinguish Escherichia from Enterobacter?


A) IMVIC
B) ONPG test
C) Citrate utilization
D) Starch hydrolysis

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Which infectious agent risk group would Mycobacterium tuberculosis be placed in?


A) Risk group 1
B) Risk group 2
C) Risk group 3
D) Risk group 4

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Which of the following is not a guideline on selection,collection,and handling of clinical specimens?


A) A small quantity of specimen should be obtained.
B) The specimen selected should be representative of the infectious disease process.
C) The specimen should be forwarded promptly to the clinical laboratory.
D) Attention must be given to avoid contamination of the specimen.

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Which of the following tests is an agglutination test for the bacterium causing typhoid fever?


A) Quellung reaction
B) API 20E system
C) Widal test
D) IMViC

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C

What is the first piece of information that is used in the identification of an unknown bacterial isolate?


A) The ability of the bacterial isolate to ferment glucose
B) The ability of the bacterial isolate to ferment lactose
C) The presence of catalase enzyme
D) The Gram stain morphology

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Which of the following is/are used to detect chlamydiae in tissues and cell scrapings?


A) PCR methods
B) Microscopy of Giemsa stained samples
C) Immunofluorescence
D) All of the choices are correct.

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What biosafety level is required when working with Staphylococcus aureus and Streptococcus pyogenes?


A) 1
B) 2
C) 3
D) 4

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Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?


A) Bright-field microscopy
B) Phase-contrast microscopy
C) Dark-field microscopy
D) DNA fingerprinting

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C

Recommended guidelines for laboratory microbiological precautions should reflect the laboratory's ________ _________.

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The major focus of the clinical microbiologist is to rapidly isolate and identify microorganisms from clinical specimens.

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All of the following methods are employed to identify pathogens in clinical specimens EXCEPT


A) special stains and microscopy.
B) culture on a variety of all-purpose, selective, and/or differential media.
C) biochemical tests.
D) molecular testing for nucleic acids.
E) immunoassays.
F) There are no exceptions here. All of these methods are used to identify pathogens.

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DNA probe technology identifies microorganisms by probing their genetic composition.

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Which of the following is not used to culture viruses?


A) Growth on artificial media
B) Growth in cell cultures
C) Growth in embryonated hen's eggs
D) Growth in whole animals

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The principle of plasmid fingerprinting is that microbial isolates of the same strain contain the same plasmids.

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________ can also be used to differentiate strains of microorganisms that differ in the antigenic composition of a structure or product.

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