Question 1

Which PCR step synthesizes complimentary DNA strands?

Accepted Answer

A)   Heat target DNA to 94° C.
B)   Add DNA polymerase and nucleotides at 72° C.
C)   Repeat the cycle of heating and cooling.
D)   Cool DNA to between 50° C and 65° C.
E)   Add primers.

Question 2

Microarray analysis is used to

Accepted Answer

A)  analyze an organism's entire genome.
B)  determine which genes are being expressed in an organism.
C)  determine the size of gene fragments after digesting with restriction endonucleases.
D)  analyze an individual's single nucleotide polymorphisms.

Question 3

Common vectors used to transfer a piece of DNA into a cloning host are

Accepted Answer

A)  plasmids.
B)  viruses.
C)  bacteriophages.
D)  artificial chromosomes.
E)  All of the choices are correct.

Question 4

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called ______.

Accepted Answer

A)  genetic engineering
B)  biotechnology
C)  recombinant DNA technology
D)  gel electrophoresis
E)  gene probe technology

Question 5

Which of the following recombinant DNA technology products is mismatched?

Accepted Answer

A)  Erythropoietin - stimulates erythrocyte production in bone marrow
B)  Factor VIII - a protein necessary for the coagulation cascade
C)  Interferons - stimulate antiviral protein production
D)  Tissue plasminogen activating factor - stimulates blood clotting

Question 6

Why is an enzyme from a thermophilic bacterium used in PCR?

Accepted Answer

A)  The enzyme makes DNA that is more similar to human DNA.
B)  This thermohile's enzyme will synthesize DNA.
C)  DNA is replicated at a high temperature that denatures most proteins.
D)  It is cheaper to obtain from live microorganisms than producing the enzyme in a lab.

Question 7

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Accepted Answer

A)  palindromes
B)  reverse transcriptases
C)  restriction endonucleases
D)  ligases
E)  DNA polymerases

Question 8

Restriction endonucleases recognize and clip DNA base sequences called ______.

Accepted Answer

A)  codons
B)  palindromes
C)  introns
D)  exons
E)  genes

Question 9

DNA strands can begin to separate at the temperature of ______.

Accepted Answer

A)  37oC
B)  42oC
C)  60oC
D)  90oC
E)  100oC

Question 10

The first genetically engineered protein approved for human use was ______.

Accepted Answer

A)  human insulin
B)  hemophilia factor VIII
C)  human adrenaline
D)  human testosterone
E)  human growth hormone

Question 11

Transformation and transduction are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following is a list of the materials required for PCR?

Accepted Answer

A)  Primers, Taq DNA polymerase, nucleotides
B)  Primers, Taq RNA polymerase, nucleotides
C)  Reverse transcriptase, Taq RNA polymerase, nucleotides
D)  Reverse transcriptase, Taq DNA polymerase, nucleotides

Question 13

Amplification of DNA is accomplished by ______.

Accepted Answer

A)  a Southern blot
B)  a Western blot
C)  DNA sequencing
D)  gene probes
E)  the polymerase chain reaction

Question 14

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Accepted Answer

A)  Pseudomonas syringae
B)  Saccharomyces cerevisiae
C)  Pseudomonas fluorescens
D)  Escherichia coli
E)  Thermus aquaticus

Question 15

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Accepted Answer

A)  Bacteria use these enzymes to attack other bacteria and destroy their DNA.
B)  They make these enzymes for humans to use in manipulating DNA.
C)  These enzymes are a defensive measure of bacteria to defend themselves against invading DNA of bacteriophages.
D)  Bacteria use these enzymes to repair their own mistakes made during DNA replication.

Question 16

What type of DNA map gives locations and sizes of DNA sections?

Accepted Answer

A)  Linkage
B)  Sequence
C)  Physical
D)  Geographical
E)  Chromosomal

Question 17

Gene probes can be labeled for detection with reporter molecules such as _____.

Accepted Answer

A)  enzymes
B)  fluorescent dyes
C)  radioisotopes
D)  All of the choices above can be used.

Question 18

Micro RNA therapy is based on the premise that

Accepted Answer

A)  	miRNA molecules function to regulate gene expression, usually by inhibiting transcription; a non-functional miRNA can be replaced thereby reducing unwanted protein production.
B)  	miRNA molecules function to regulate gene expression, usually by promoting translation; a non-functional miRNA can be repaired thereby reducing unwanted protein production.
C)  	miRNA molecules function to regulate gene expression, usually by initiating transcription; an over-production of miRNA can be replaced thereby promoting necessary protein production.
D)  	miRNA molecules function to regulate protein production, usually by inhibiting translation; a hyperactive miRNA can be replaced thereby reducing unwanted proteins.

Question 19

Each of the following are features of a cloning host except

Accepted Answer

A)  quick generation time.
B)  minimal growth requirements.
C)  mapped genome.
D)  pathogenicity.
E)  transformable.

Question 20

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering and Recombinant Dna

Restriction endonucleases recognize and clip DNA base sequences called ______.

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with reporter molecules such as _____.

Correct AnswerverifiedShow Answer

Why is an enzyme from a thermophilic bacterium used in PCR?

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Correct AnswerverifiedShow Answer

DNA strands can begin to separate at the temperature of ______.

Correct AnswerverifiedShow Answer

Transformation and transduction are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and its subsequent transfer into the genome of another organism is a specific technique called ______.

Correct AnswerverifiedShow Answer

Micro RNA therapy is based on the premise that

Correct AnswerverifiedShow Answer

Microarray analysis is used to

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complimentary DNA strands?

Correct AnswerverifiedShow Answer

Each of the following are features of a cloning host except

Correct AnswerverifiedShow Answer

Which of the following recombinant DNA technology products is mismatched?

Correct AnswerverifiedShow Answer

Amplification of DNA is accomplished by ______.

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct AnswerverifiedShow Answer

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Correct AnswerverifiedShow Answer

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Correct AnswerverifiedShow Answer

The first genetically engineered protein approved for human use was ______.

Correct AnswerverifiedShow Answer

Common vectors used to transfer a piece of DNA into a cloning host are

Correct AnswerverifiedShow Answer

What type of DNA map gives locations and sizes of DNA sections?

Correct AnswerverifiedShow Answer

Which of the following is a list of the materials required for PCR?

Correct AnswerverifiedShow Answer

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