Question 1

Which of the following is correct concerning F factors?

Accepted Answer

A)   It may integrate into the host chromosome
B)   It stands for fertility factor
C)   It allows conjugation to occur
D)   F+ bacteria act as donor strains in conjugation
E)   All of the answers are correct

Question 2

Which of the following conditions must be correct for transformation to occur?

Accepted Answer

A)   Temperature
B)   Ionic conditions
C)   Correct nutrient balance
D)   All of the answers are correct

Question 3

In the experiment performed by Lederberg and Tatum bacteria of different genotypes had to be mixed together to obtain genetic transfer. However, Davis performed the experiment in a slightly different manner by separating cells with a filter incapable of allowing bacteria to pass. What possibility did the experiment by Davis exclude that could have occurred by the protocol used by Lederberg and Tatum?

Accepted Answer

A)   transformation
B)   conjugation
C)   generalized transduction
D)   specialized transduction

Question 4

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA)  directly from the environment?

Accepted Answer

A)   Transformation
B)   Transduction
C)   Conjugation
D)   All of the answers are correct

Question 6

Competent cells are those used in studies of conjugation.

Accepted Answer

A) True 
 B)False

Question 7

Which mechanism of genetic transfer utilizes a sex pilus?

Accepted Answer

A)   Transformation
B)   Transduction
C)   Conjugation
D)   All of the answers are correct

Question 8

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Accepted Answer

A)   It serves as a control to calculate the rate of reversion mutations
B)   There is no experimental purpose to performing that part of the experiment
C)   It tests that the strains were made correctly
D)   It tests to make sure the media was made correctly

Question 9

Although there are several different types of plasmids they all

Accepted Answer

A)   can confer some kind of selective growth advantage depending on environmental conditions.
B)   can induce a mating phenotype.
C)   result in horizontal transfer.
D)   make cells resistant to antibiotics

Question 10

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Accepted Answer

A) True 
 B)False

Question 11

What is the correct order for the steps in transduction?

Accepted Answer

A)   1)  Phage infects cell 2)  Phage transported DNA recombines with chromosome of new cell 3)  Host DNA digested into fragments 4)  Some phage accidently carry host DNA fragment 5)  Phage infects new cell
B)   1)  Phage infects cell 2)  Phage transported DNA recombines with chromosome of new cell 3)  Host DNA digested into fragments 4)  Phage infects new cell 5)  Some phage accidently carry host DNA fragment
C)   1)  Phage infects cell 2)  Host DNA digested into fragments 3)  Some phage accidently carry host DNA fragment 4)  Phage infects new cell 5)  Phage transported DNA recombines with chromosome of new cell
D)   1)  Phage infects cell 2)  Phage transported DNA recombines with chromosome of new cell 3)  Some phage accidently carry host DNA fragment 4)  Phage infects new cell 5)  Host DNA digested into fragments

Question 12

Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Accepted Answer

A) True 
 B)False

Question 13

Mechanism of genetic transfer that uses competent cells.

Accepted Answer

A)   Transformation
B)   Transduction
C)   Conjugation
D)   All of the answers are correct

Question 14

Which statement is true concerning intragenic and intergenic gene mapping?

Accepted Answer

A)   The number of expected wild type recombinant viruses will be greater in intragenic mapping than with intergenic mapping
B)   Intergenic gene mapping can only be performed in viruses.It is not performed in bacteria while intragenic mapping is performed in both viruses and bacteria
C)   Intragenic mapping is the mapping of mutations within a gene while intergenic mapping is the mapping of genes with respect to each other
D)   Intergenic mapping is the mapping of mutations within a gene while intragenic mapping is the mapping of genes with respect to each other

Question 15

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Accepted Answer

A)   Raise the cotransduction frequency
B)   Lower the cotransduction frequency
C)   Have no effect on the cotransduction frequency

Question 16

Which of the following is true regarding conjugation?

Accepted Answer

A)   The bacteria must be in physical contact
B)   Most bacterial species have the ability to conjugate
C)   Strains called donor strains can transfer genetic information
D)   Donor strains can convert non-donor strains into donor strains
E)   All of the answers are correct

Question 17

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68?

Accepted Answer

A)   0.68=(1 - d/2) 3
B)   d=.683 - 1
C)   d=(1-.68) 2

Question 18

Based on the following data, what two genes are farthest apart?

Accepted Answer

A)   a-b
B)   b-c
C)   a-c

Question 19

The protein complex that initiates the process of conjugation following contact between two bacteria is called the _________.

Accepted Answer

A)   origin of transfer
B)   T DNA
C)   relaxosome
D)   nucleoprotein

Exam 7: Genetic Transfer and Mapping in Bacteria and Bacteriophages

Which mechanism of genetic transfer utilizes a sex pilus?

Correct Answer
verified

Although there are several different types of plasmids they all

Correct Answer
verified

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68?

Correct Answer
verified

Which of the following is true regarding conjugation?

Correct Answer
verified

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Correct Answer
verified

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Correct Answer
verified

Which of the following conditions must be correct for transformation to occur?

Correct Answer
verified

The protein complex that initiates the process of conjugation following contact between two bacteria is called the _________.

Correct Answer
verified

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Correct Answer
verified

Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Correct Answer
verified

What is the correct order for the steps in transduction?

Correct Answer
verified

Mechanism of genetic transfer that uses competent cells.

Correct Answer
verified

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

Correct Answer
verified

Correct Answer
verified

Competent cells are those used in studies of conjugation.

Correct Answer
verified

Which of the following is correct concerning F factors?

Correct Answer
verified

Based on the following data, what two genes are farthest apart?

Correct Answer
verified

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Correct Answer
verified

Which statement is true concerning intragenic and intergenic gene mapping?

Correct Answer
verified

Exam 7: Genetic Transfer and Mapping in Bacteria and Bacteriophages

Which mechanism of genetic transfer utilizes a sex pilus?

Correct AnswerverifiedShow Answer

Although there are several different types of plasmids they all

Correct AnswerverifiedShow Answer

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68?

Correct AnswerverifiedShow Answer

Which of the following is true regarding conjugation?

Correct AnswerverifiedShow Answer

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Correct AnswerverifiedShow Answer

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Correct AnswerverifiedShow Answer

Which of the following conditions must be correct for transformation to occur?

Correct AnswerverifiedShow Answer

The protein complex that initiates the process of conjugation following contact between two bacteria is called the _________.

Correct AnswerverifiedShow Answer

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Correct AnswerverifiedShow Answer

Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Correct AnswerverifiedShow Answer

What is the correct order for the steps in transduction?

Correct AnswerverifiedShow Answer

Mechanism of genetic transfer that uses competent cells.

Correct AnswerverifiedShow Answer

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Competent cells are those used in studies of conjugation.

Correct AnswerverifiedShow Answer

Which of the following is correct concerning F factors?

Correct AnswerverifiedShow Answer

Based on the following data, what two genes are farthest apart?

Correct AnswerverifiedShow Answer

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Correct AnswerverifiedShow Answer

Which statement is true concerning intragenic and intergenic gene mapping?

Correct AnswerverifiedShow Answer

Correct Answer
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