Question 1

Which of the following is most similar to lysogeny?

Accepted Answer

A)   Hfr state
B)   F⁺ state
C)   F⁻ state
D)   F' state

Question 2

The killing of cells by UV irradiation involves

Accepted Answer

A)   absorption at 260 nm by proteins only.
B)   absorption at 260 nm by RNA only.
C)   formation of pyrimidine dimers.
D)   formation of purine dimers.

Question 3

Insertion sequences are found on both ends of transposons and encode for transposase.

Accepted Answer

A) True 
 B)False

Question 4

Why is the outcome of a base-pair substitution mutation variable? List and describe the possible outcomes of a base-pair substitution mutation in your answer.

Accepted Answer

The outcome of a base-pair substitution ...

Question 5

Mutation rates are similar in Bacteria and Archaea,yet certain stressful conditions mutation rates increase.Why is the mutation rate not constant and close to zero all of the time?

Accepted Answer

A)   Increased mutation rates can be advantageous in rapidly changing environments because some random mutations may be useful for survival.
B)   Microorganisms carefully control the mutation rate of their DNA to match the environmental conditions and maximize evolution.
C)   The increased mutation rate under stressful conditions is an indication that the microorganisms can no longer replicate their DNA properly and are about to die.
D)   Constant mutation rates would halt evolution completely.

Question 6

A deleterious mutation in recA results in

Accepted Answer

A)   a decrease in specific recombination.
B)   a decrease in homologous recombination.
C)   an increase in homologous recombination.
D)   no change in either general or specific recombination.

Question 7

Lysogeny probably carries a strong selective advantage for the host cell because it

Accepted Answer

A)   prevents cell lysis.
B)   confers resistance to infection by viruses of the same type.
C)   confers resistance to infection by viruses of a different type (or strain) .
D)   confers resistance to infection by many virus types and prevent cell lysis.

Question 8

A mutant that has a nutritional requirement for growth is an example of a(n)

Accepted Answer

A)   autotroph.
B)   auxotroph.
C)   heterotroph.
D)   organotroph.

Question 9

When the F factor is integrated,all Hfr strains have the origin of replication functioning in the same direction.

Accepted Answer

A) True 
 B)False

Question 10

F⁻ strains of Escherichia coli

Accepted Answer

A)   do not have an F factor.
B)   have the F factor as a plasmid.
C)   have an integrated F factor.
D)   transfer the F factor to other strains at a high frequency.

Question 11

The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's

Accepted Answer

A)   plasmid type.
B)   genotype.
C)   phenotype.
D)   mutation type.

Question 12

Which of the following methods may introduce foreign DNA into a recipient?

Accepted Answer

A)   transformation
B)   transduction
C)   conjugation
D)   transformation, transduction, and conjugation

Question 13

Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?

Accepted Answer

A)   conjugation of an F+ plasmid
B)   gene transfer through a genetic transfer agent
C)   transduction by a dsDNA phage Mu
D)   transformation of a linear piece of DNA

Question 14

How might mutations in the dnaQ sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

Accepted Answer

Mutations in dnaQ would result in an inc...

Question 15

Integration of linear transforming DNA into the chromosome

Accepted Answer

A)   is not required for the expression for the transformed genes.
B)   is catalyzed by the RecA gene.
C)   almost never occurs because restriction endonuclease will degrade the DNA before it is integrated.
D)   only occurs in laboratory-based systems in artificial competent cells.

Question 16

Many Bacteria isolated from nature are natural lysogens.

Accepted Answer

A) True 
 B)False

Question 17

Hfr strains of Escherichia coli

Accepted Answer

A)   do not possess an F factor.
B)   have the F factor as a plasmid.
C)   have an integrated F factor.
D)   transfer the complete F factor to recipient cells at a high frequency.

Question 18

The production of a functional gene product by transforming bacteria that lack a lacZ gene with a plasmid containing a lacZ gene is known as

Accepted Answer

A)   complementation.
B)   mitosis.
C)   transfection.
D)   reversion.

Question 19

A mutant you made in the laboratory with 5-bromouracil suddenly regains the wild-type phenotype.You discuss this phenomenon with your advisor and colleagues,and they suggest you try transposon mutagenesis to avoid this problem and create stable mutants.Why would mutants created with 5-bromouracil be more likely to regain the wild-type phenotype than mutants created via transposon mutagenesis?

Accepted Answer

Because a point mutation involves only a...

Question 20

Genetic recombination involving insertion sequences typically results in what type of mutation?

Accepted Answer

A)   base-pair substitution mutation
B)   silent mutation
C)   frameshift mutation
D)   base-pair deletion mutation

Exam 10: Genetics of Bacteria and Archaea

A deleterious mutation in recA results in

Correct Answer
verified

Why is the outcome of a base-pair substitution mutation variable? List and describe the possible outcomes of a base-pair substitution mutation in your answer.

Correct Answer
verified
The outcome of a base-pair substitution ...
View Answer

Which of the following is most similar to lysogeny?

Correct Answer
verified

F⁻ strains of Escherichia coli

Correct Answer
verified

Lysogeny probably carries a strong selective advantage for the host cell because it

Correct Answer
verified

Genetic recombination involving insertion sequences typically results in what type of mutation?

Correct Answer
verified

A mutant that has a nutritional requirement for growth is an example of a(n)

Correct Answer
verified

Hfr strains of Escherichia coli

Correct Answer
verified

Integration of linear transforming DNA into the chromosome

Correct Answer
verified

The production of a functional gene product by transforming bacteria that lack a lacZ gene with a plasmid containing a lacZ gene is known as

Correct Answer
verified

Insertion sequences are found on both ends of transposons and encode for transposase.

Correct Answer
verified

Which of the following methods may introduce foreign DNA into a recipient?

Correct Answer
verified

When the F factor is integrated,all Hfr strains have the origin of replication functioning in the same direction.

Correct Answer
verified

The killing of cells by UV irradiation involves

Correct Answer
verified

Many Bacteria isolated from nature are natural lysogens.

Correct Answer
verified

Correct Answer
verified
Because a point mutation involves only a...
View Answer

The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's

Correct Answer
verified

Mutation rates are similar in Bacteria and Archaea,yet certain stressful conditions mutation rates increase.Why is the mutation rate not constant and close to zero all of the time?

Correct Answer
verified

How might mutations in the dnaQ sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

Correct Answer
verified
Mutations in dnaQ would result in an inc...
View Answer

Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?

Correct Answer
verified

Exam 10: Genetics of Bacteria and Archaea

A deleterious mutation in recA results in

Correct AnswerverifiedShow Answer

Why is the outcome of a base-pair substitution mutation variable? List and describe the possible outcomes of a base-pair substitution mutation in your answer.

Correct AnswerverifiedThe outcome of a base-pair substitution ...View AnswerShow Answer

Which of the following is most similar to lysogeny?

Correct AnswerverifiedShow Answer

F⁻ strains of Escherichia coli

Correct AnswerverifiedShow Answer

Lysogeny probably carries a strong selective advantage for the host cell because it

Correct AnswerverifiedShow Answer

Genetic recombination involving insertion sequences typically results in what type of mutation?

Correct AnswerverifiedShow Answer

A mutant that has a nutritional requirement for growth is an example of a(n)

Correct AnswerverifiedShow Answer

Hfr strains of Escherichia coli

Correct AnswerverifiedShow Answer

Integration of linear transforming DNA into the chromosome

Correct AnswerverifiedShow Answer

The production of a functional gene product by transforming bacteria that lack a lacZ gene with a plasmid containing a lacZ gene is known as

Correct AnswerverifiedShow Answer

Insertion sequences are found on both ends of transposons and encode for transposase.

Correct AnswerverifiedShow Answer

Which of the following methods may introduce foreign DNA into a recipient?

Correct AnswerverifiedShow Answer

When the F factor is integrated,all Hfr strains have the origin of replication functioning in the same direction.

Correct AnswerverifiedShow Answer

The killing of cells by UV irradiation involves

Correct AnswerverifiedShow Answer

Many Bacteria isolated from nature are natural lysogens.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedBecause a point mutation involves only a...View AnswerShow Answer

The designations Phe⁻,Leu⁻,and Ser⁺ refer to an organism's

Correct AnswerverifiedShow Answer

Mutation rates are similar in Bacteria and Archaea,yet certain stressful conditions mutation rates increase.Why is the mutation rate not constant and close to zero all of the time?

Correct AnswerverifiedShow Answer

How might mutations in the dnaQ sequence,whose enzyme is involved in DNA proofreading,be beneficial for a microbe in a highly competitive and dynamic community?

Correct AnswerverifiedMutations in dnaQ would result in an inc...View AnswerShow Answer

Which process listed below does NOT require homologous recombination to maintain the new genetic material in the recipient cell?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
The outcome of a base-pair substitution ...
View Answer

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verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Because a point mutation involves only a...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Mutations in dnaQ would result in an inc...
View Answer

Correct Answer
verified