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Green fluorescent protein (GFP) can be used to fluorescently label a specific protein in cells by genetically engineering cells to synthesize the target protein fused to GFP. What is the advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein?


A) GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires preserved cells.
B) GFP fusions enable higher resolution than staining with fluorescent probes.
C) GFP permits the position of the protein in the cell more precisely than fluorescent probes.
D) GFP permits visualization of protein-protein interactions; fluorescent probes do not.
E) GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.

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The minimum distance two points can be separated and still discerned as separate is the


A) resolution.
B) magnification.
C) visibility.
D) objective magnification.
E) contrast.

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The difference in lipid and protein composition between the membranes of the endomembrane system is largely determined by


A) the physical separation of most membranes from each other.
B) the transportation of membrane lipids among the endomembrane system by small membrane vesicles.
C) the function of the Golgi apparatus in sorting and directing membrane components.
D) the modification of the membrane components once they reach their final destination.
E) the synthesis of different lipids and proteins in each of the organelles of the endomembrane system.

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Which cell would be best for studying lyosomes?


A) muscle cell
B) nerve cell
C) phagocytic white blood cell
D) leaf cell of a plant
E) bacterial cell

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Use the following figure to answer the questions below. Use the following figure to answer the questions below.    -The structure labelled D functions in A) lipid synthesis. B) membrane production. C) cytosolic protein production. D) modification of proteins for transport. E) package of proteins. -The structure labelled D functions in


A) lipid synthesis.
B) membrane production.
C) cytosolic protein production.
D) modification of proteins for transport.
E) package of proteins.

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A mycoplasma is an organism with a diameter between 0.1 and 1.0 µm. What does the organism's size tell you about how it might be classified?


A) It must be a single-celled protist.
B) It must be a single-celled fungus.
C) It could be almost any typical bacterium.
D) It could be a typical virus.
E) It could be a very small bacterium.

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What technique would be most appropriate to use to observe the movements of condensed chromosomes during cell division?


A) light microscopy
B) scanning electron microscopy
C) transmission electron microscopy
D) confocal fluorescence microscopy
E) super-resolution fluorescence microscopy

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Use the following figure to answer the questions below. Use the following figure to answer the questions below.    -What is the name of the structure labelled B? A) cell wall B) plasma membrane C) nuclear envelope D) cytosol E) endoplasmic reticulum -What is the name of the structure labelled B?


A) cell wall
B) plasma membrane
C) nuclear envelope
D) cytosol
E) endoplasmic reticulum

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A newspaper ad for a local toy store indicates that a very inexpensive microscope available for a small child is able to magnify specimens nearly as much as the much more costly microscope available in your college lab. What is the primary reason for the price difference?


A) The ad agency is misrepresenting the ability of the toy microscope to magnify.
B) The toy microscope does not have the same fine control for focus of the specimen.
C) The toy microscope magnifies a good deal, but has low resolution and therefore poor quality images.
D) The college microscope produces greater contrast in the specimens.
E) The toy microscope usually uses a different wavelength of light source.

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Cyanide binds with at least one molecule involved in producing ATP. If a cell is exposed to cyanide, most of the cyanide will be found within the


A) mitochondria.
B) ribosomes.
C) peroxisomes.
D) lysosomes.
E) endoplasmic reticulum.

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When biologists wish to study the internal ultrastructure of cells, they can achieve the finest resolution by using


A) a phase-contrast light microscope.
B) a scanning electron microscope.
C) a transmission electronic microscope.
D) a confocal fluorescence microscope.
E) a super-resolution fluorescence microscope.

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What types of proteins are not synthesized in the rough ER?


A) endoplasmic reticulum proteins
B) extracellular matrix proteins
C) secreted proteins
D) mitochondrial proteins
E) plasma membrane proteins

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Why isn't the mitochondrion classified as part of the endomembrane system?


A) It is a static structure.
B) Its structure is not derived from the ER or Golgi.
C) It has too many vesicles.
D) It is not involved in protein synthesis.
E) It is not attached to the outer nuclear envelope.

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Which of the following is present in a prokaryotic cell?


A) mitochondrion
B) ribosome
C) nuclear envelope
D) chloroplast
E) ER

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The Golgi apparatus has a polarity or sidedness to its structure and function. Which of the following statements correctly describes this polarity?


A) Transport vesicles fuse with one side of the Golgi and leave from the opposite side.
B) Proteins in the membrane of the Golgi may be sorted and modified as they move from one side of the Golgi to the other.
C) Lipids in the membrane of the Golgi may be sorted and modified as they move from one side of the Golgi to the other.
D) Soluble proteins in the cisternae (interior) of the Golgi may be sorted and modified as they move from one side of the Golgi to the other.
E) All of the above correctly describe polar characteristics of the Golgi function.

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What is the most likely pathway taken by a newly synthesized protein that will be secreted by a cell?


A) ER → Golgi → nucleus
B) Golgi →ER →lysosome
C) nucleus →ER →Golgi
D) ER →Golgi →vesicles that fuse with plasma membrane
E) ER →lysosomes →vesicles that fuse with plasma membrane

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Which of the following is a major cause of the size limits for certain types of cells?


A) limitation on the strength and integrity of the plasma membrane as cell size increases
B) the difference in plasma membranes between prokaryotes and eukaryotes
C) evolutionary progression in cell size; more primitive cells have smaller sizes
D) the need for a surface area of sufficient area to support the cell's metabolic needs
E) rigid cell walls that limit cell size expansion

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Which of the following produces and modifies polysaccharides that will be secreted?


A) lysosome
B) vacuole
C) mitochondrion
D) Golgi apparatus
E) peroxisome

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Tay-Sachs disease is a human genetic abnormality that results in cells accumulating and becoming clogged with very large and complex lipids. Which cellular organelle must be involved in this condition?


A) the endoplasmic reticulum
B) the Golgi apparatus
C) the lysosome
D) mitochondria
E) membrane-bound ribosomes

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Which type of organelle is found in plant cells but not in animal cells?


A) ribosomes
B) mitochondria
C) nuclei
D) plastids
E) none of these

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