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Exam 20: Genomics

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Question 1

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Why are transposable elements considered to be selfish genes?

A) They produce products toxic to the host.
B) They replicate outside of the chromosome.
C) They replicate using the host's resources without direct benefit to the host.
D) They spread rapidly throughout the genome.
E) They produce products that prevent cooperation between cells.

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Question 2

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If one wished to test the hypothesis that humans and chimps differ due to differences in the expression of a large set of shared genes,the technique to use would be

A) Southern blotting.
B) PCR.
C) DNA sequencing.
D) protein-protein interaction assays.
E) DNA microarray analysis.

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Question 3

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In comparing DNA sequences from two different eukaryotic organisms,which do you expect to vary the most?

Which of the following questions is asked in functional genomics research?

In establishing paternity by DNA fingerprinting,what proportion of microsatellite repeat alleles in a child come from the father?

Like DNA microarrays,Northern blots analyze the levels of mRNA.However,Northern blots are almost always prepared with a probe tagged with one label,whereas DNA microarrays almost always use two groups of probes,each one tagged with a different fluorescent label.What advantage is conferred by using two labels for the microarray probes?

Although transposable elements and simple tandem repeats (STRs) are both repetitive DNAs,they differ in that

The discipline that manages,analyzes,and interprets the vast amounts of sequence data generated from whole genome sequencing is

The goal of annotating a whole genome sequence is to

What can proteomics reveal that genomics cannot?

One surprising discovery from the analysis of bacterial genomes is that

Table 20.1 The following table from the Venter et al. paper presents the numbers of different types of genes discovered in this study. -How do you think putative genes were placed into the categories shown in the preceding table?

When gene duplication occurs to its ultimate extent by doubling all genes in a genome,what has occurred?

Imagine that your goal is to isolate mouse mutants that stabilize microsatellite repeat sequences (i.e.,lower the frequency at which their repeat number changes) .Which of the following mutations would you predict to have this stabilizing effect?

Sequencing eukaryotic genomes is more difficult than sequencing genomes of bacteria or archaea because of

As with many new endeavors,concerns have been raised by some that genomics will fundamentally change the way biological research is practiced.Examine the full reference for the Venter et al.paper and consider the way this work was done.Now think of some seminal works in the history of biology,for example,Darwin's On the Origin of Species and Watson and Crick's publication on DNA structure.What do you think the concern might be that has been voiced about genomics?

If alternative splicing did not occur,a prediction would be that

If practical applications arise from this or similar metagenomic studies,what might these be?

Figure 20.1 -The figure above compares the proportion of genes devoted to various functions in a set of model organisms.Based on this figure,which one of the following statements is FALSE?

Figure 20.2 -In the microarray shown in the figure above,if you were searching for genes whose expression was inhibited by hormone treatment,you would search for sequences spotted on the array that showed ________ fluorescence.