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Figure 13-3 Figure 13-3   Refer to the accompanying figure. Which label indicates the location of the promoter region? A)  A B)  B C)  C D)  D E)  E Refer to the accompanying figure. Which label indicates the location of the promoter region?


A) A
B) B
C) C
D) D
E) E

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Figure 13-2 Figure 13-2   Refer to the accompanying figure. The portion of the molecule labeled as 5 is: A)  the attached amino acid. B)  a double-stranded DNA region. C)  a single-stranded DNA region. D)  the anticodon. E)  the codon. Refer to the accompanying figure. The portion of the molecule labeled as 5 is:


A) the attached amino acid.
B) a double-stranded DNA region.
C) a single-stranded DNA region.
D) the anticodon.
E) the codon.

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Draw a general overview of the flow of genetic information from DNA to protein in eukaryote vs. prokarytotes. You should construct one drawing for eukaryotes and one for prokaryotes. Label all structures, molecules, processes, and cellular compartments.

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The flow d...

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In the Beadle and Tatum study, it was important that Neurospora was haploid because:


A) it is easier to grow haploid molds in the laboratory.
B) haploid molds have simpler nutritional requirements than do diploid molds.
C) a mutation that arises is not masked by a normal allele on a homologous chromosome.
D) haploid Neurospora will always mutate.
E) diploid Neurospora will always mutate.

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snRNPs function to splice introns together. __________________

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These noncoding sequences located within coding regions of ____ genes are called ____.


A) only eukaryotic; introns
B) eukaryotic and prokaryotic; introns
C) only prokaryotic; introns
D) only eukaryotic; exons
E) only prokaryotic; exons

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What evolutionary role is served by the redundancy of the genetic code?

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Concepts to Consider: The redundancy of ...

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A polyribosome is:


A) a complex composed of several ribosomes and plus an mRNA transcript.
B) a complex componsed of several ribosomes in eukaryotes.
C) an initiation complex in eukaryotes.
D) an elongation complex in eukaryotes.
E) one ribosome complexed to several mRNAs.

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How is the four-letter language of nucleic acids converted into the twenty-word language of amino acids?


A) The four nucleic acid bases combine in two-letter combinations that define different amino acids.
B) The four nucleic acid bases combine in three-letter sequences that define different amino acids.
C) Triplets of the two-letter nucleic acid bases are translated into the twenty different amino acids.
D) The four bases each specify one amino acid, which in combination give rise to the remaining sixteen amino acids.
E) The four bases are first converted into tRNA molecules, which can each attach to five amino acids.

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The 3' trailing sequences are located upstream of the stop codon. __________________

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Reverse transcriptase catalyzes the synthesis of DNA from an RNA template. __________________

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Eukaryotic mRNA has a significantly longer half life than its prokaryotic counterpart. How is this accomplished in eukaryotes? What processes tend to degrade mRNA molecules in both eukaryotes and prokaryotes, and why are these processes needed?

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Concepts to Consider: Eukaryotic mRNA is...

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Following peptide bond formation between the amino acid in the A site on the ribosome and the growing polypeptide chain, the tRNA in the A site:


A) releases the growing polypeptide chain.
B) picks up another amino acid to add to the chain.
C) moves to the P site of the ribosome.
D) forms a peptide bond with A site of the ribosome.
E) forms a covalent bond with the P site of the ribosome.

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RNA polymerase binds to the promoter sequence in RNA . __________________

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If an mRNA transcript of the human XYZ gene was introduced into a yeast cell, the transcript would be:


A) degraded immediately.
B) translated into a yeast-specific gene.
C) translated into the human XYZ protein.
D) exported outside the cell.
E) transcribed into the XYZ mRNA.

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Match the event to the correct process of protein synthesis.

Premises
growth of the polypeptide chain
the first stage of translation
triggered by a stop codon
protein synthesis proceeding in a 5' to 3' direction
assembly of the ribosome
completed polypeptide chain released from ribosome
Responses
elongation
initiation
termination

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growth of the polypeptide chain
the first stage of translation
triggered by a stop codon
protein synthesis proceeding in a 5' to 3' direction
assembly of the ribosome
completed polypeptide chain released from ribosome

Leader sequences contain signals that:


A) prevent enzymes from degrading the newly synthesized mRNA.
B) inhibit ribosome binding until the appropriate time.
C) initiate chain termination.
D) allow the ribosomes to be properly positioned for translation of mRNA molecules.
E) allow tRNA molecules to successfully bind to mRNA.

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Discuss which type of DNA mutation in the coding region of an essential human gene (for example, insulin) would be the most harmful to a phenotype. Consider missense, nonsense, frameshifts, and transposons.

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Answers can vary, but missense mutations...

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Match the RNA molecule with its corresponding description.

Premises
small double-stranded RNA molecules; regulates gene expression
large RNA molecules; regulates chromatin structure
small RNA molecules; inhibits mobile DNA element activity in germ cells
small RNA molecules; inhibit translation
helps to deliver the ribosome-mRNA-polypeptide complex to the rough ER
responsible for pre-rRNA molecule processing in the nucleolus
Responses
SRP RNA
miRNA
snoRNA
siRNA
lncRNA
piRNA

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small double-stranded RNA molecules; regulates gene expression
large RNA molecules; regulates chromatin structure
small RNA molecules; inhibits mobile DNA element activity in germ cells
small RNA molecules; inhibit translation
helps to deliver the ribosome-mRNA-polypeptide complex to the rough ER
responsible for pre-rRNA molecule processing in the nucleolus

Beadle and Tatum began their studies with wild-type Neurospora. How is this organism best characterized?


A) A strain that only grows in the wild
B) A mutant strain that will only grow in the lab on complete medium
C) A strain that will not grow in the lab
D) A virulent strain
E) A normal phenotype that will grow on minimal medium

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