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Protons are pumped by mitochondria during active electron transport


A) into the thylakoid lumen.
B) outside the outer mitochondrial membrane.
C) into the mitochondrial matrix.
D) outside the inner mitochondrial membrane.

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The electron transport chain component that transfers electrons directly to oxygen is


A) complex I.
B) cytochrome c.
C) complex IV.
D) NADH.

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For one electron entering the electron transport chain at complex I, how many times is it handed off between redox active cofactors on its way through the enzyme complexes to end up on O2?


A) ~2000
B) ~200
C) ~20
D) ~2

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Which statement comparing chloroplasts and mitochondria is true?


A) Chloroplasts and mitochondria each contain two membranes.
B) Chloroplasts and mitochondria use electrons from NADH to create a proton gradient.
C) Chloroplasts pump protons outside the organelle, while mitochondria pump protons inside the inner part of the organelle.
D) Chloroplasts and mitochondria use energy from a proton gradient to make ATP.

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The __________ causes the catalytic headpiece of ATP synthase to change conformation.


A) rotation of the circle of the α\alpha 3 β\beta 3 subunits
B) movement of the circle of ~10 c subunits
C) interaction with the rotating central γ\gamma subunit
D) binding of ADP and Pi

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Explain how ATP synthase can be used as primary active transporter of H+ and pump protons.

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ATP synthase can be run in the reverse d...

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Complex IV in the mitochondrial electron transport chain belongs to which enzyme class?


A) lyase
B) hydrolase
C) transferase
D) oxidoreductase

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How are many mitochondrial diseases passed on from parents to offspring?


A) through the mother only
B) through the father only
C) through an equal distribution from both parents
D) by random mutations in the mitochondrial genome

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Thermogenin (or uncoupling protein) is not toxic like 2,4-dinitrophenol because the protein


A) is typically expressed in adipose tissues where ample fat calories are found.
B) is less efficient at proton dissipation.
C) cannot leave the mitochondria.
D) is naturally occurring and not synthetic.

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Which statement is true of the mitochondrial electron transport system?


A) All the electron carriers are located in enzyme complexes.
B) All the electrons in the chain end up on O2 to produce water.
C) The pH drops in the mitochondria as electrons pass through the system.
D) Protons are pumped from the inner membrane space to the mitochondrial matrix during the electron transfer.

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Explain why many mitochondrial diseases are inherited from the mother only.

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Many of the proteins in the mitochondria...

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What is the reaction catalyzed by complex III in the electron transport system?


A) UQH2 + O2 \rightarrow UQ + H2O
B) UQH2 + complex IV+ \rightarrow UQ + complex IV
C) FADH2 + UQ \rightarrow FAD + UQH2
D) UQH2 + cytochrome c+ \rightarrow UQ + cytochrome c

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On the left hand side of the figure, label the regions above and below the lipid membrane assuming the process occurs in mitochondria. On the right hand side, label the regions assuming the process occurs in chloroplasts. On the left hand side of the figure, label the regions above and below the lipid membrane assuming the process occurs in mitochondria. On the right hand side, label the regions assuming the process occurs in chloroplasts.

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Which of the following statements about the chemiosmotic theory is true?


A) It requires an enclosed mitochondrial membrane.
B) The membrane ATPase (or ATP synthase) has no significant role in the theory.
C) Energy is coupled through a transmembrane electron gradient.
D) It explains how ATP energy is used to create a pH gradient.

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Use the following picture of a mitochondrion and label the (A) matrix, (B) crista, (c) inner membrane space, (D) cytoplasm, (E) inner membrane, (F) outer membrane, (G) location of the ATP synthesis, (H) location of elevated pH, and (I) the location of the electron transport system complexes. Use the following picture of a mitochondrion and label the (A) matrix, (B) crista, (c) inner membrane space, (D) cytoplasm, (E) inner membrane, (F) outer membrane, (G) location of the ATP synthesis, (H) location of elevated pH, and (I) the location of the electron transport system complexes.

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blured image In addition, (D) the cytoplasm is outsi...

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A decrease in would be LEAST likely to affect the processes of the electron transport system.


A) oxygen concentrations in the cell
B) the TCA cycle activity
C) cellular CO2 concentrations
D) the concentration of cellular NADH

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List the order of enzymes, enzyme complexes, and membrane soluble electron carriers that an electron is passed through on its way from NADH to O2 in the electron transport system.

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NADH blured image complex I blured image ubi...

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Explain the origin of the brown color of brown adipose tissue.

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Brown adipose tissue is brown ...

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ATP synthase is located in or on the


A) cytoplasm.
B) intermembrane space of the mitochondria.
C) inner mitochondrial membrane.
D) outer mitochondrial membrane.

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Protons in the mitochondria are


A) the driving force for the electron transfers in the electron transport system.
B) pumped by mitochondrial electron transport system enzymes.
C) pumped inside the mitochondria using ATP energy.
D) the cause of a lower pH inside the mitochondria than outside the mitochondria.

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