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Exam 6: Microbial Growth

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Question 21

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A culture medium consisting of agar, peptone, and beef heart is a

A) chemically defined medium.
B) reducing medium.
C) selective medium.
D) complex medium.
E) differential medium.

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Question 22

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Figure 6.1 -In Figure 6.1, which line best depicts a facultative anaerobe in the absence of O₂?

A) a
B) b
C) c

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Question 23

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Most pathogenic bacteria are thermophiles.

Which enzyme catalyzes the reaction: 2H₂O2 -2H₂O + O₂?

Which of the following is NOT a direct method to measure microbial growth?

Which of the following elements is NOT correctly matched with its cellular function?

If cells are grown in media containing amino acids labeled with radioactive nitrogen (¹⁵N) , most of radioactivity will be found in the cells'

Which enzyme catalyzes the reaction: O₂- + O₂- + 2H⁺ -H₂O₂ + O₂?

Which of the following pairs is mismatched?

Most fungi grow best at pH

Pure cultures can easily be obtained on streak plates, even if the desired bacteria are present in very low concentrations.

Table 6.2 The following data show growth of two bacteria on different media. $\begin{array}{l}\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\quad\text { Amount of Growth }\\\begin{array} { | l | c | c | } \hline & \text { Staphylococcus avoeus } & \text { Streptococcus pyogenes } \\\hline \text { Nutrient agar } & + + & + + \\\hline \text { Nutrient agar + 7.5\% NaCl } & + & - \\\hline\end{array}\end{array}$ -The data in Table 6.2 indicate that S. aureus is a(n)

An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through?

Which group of microorganisms is most likely to spoil a freshwater trout preserved with salt?

Table 6.1 Three different culture media are shown below. $\begin{array} { l l l } \text { Medium A } & \text { Medium B } & \text { Medium } \mathrm { C } \\\mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Tide detergent } & \text { Glucose } \\\mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Peptone } \\\mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } \\\mathrm { CaCl } _ { 2 } & \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 2 } \\\mathrm { NaHCO } _ { 3 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 }\end{array}$ -In Table, 6.1, in which medium (or media) would an autotroph grow?

Figure 6.3 -In Figure 6.3, which tube shows the expected growth pattern for a microaerophile?

Which of the following is an advantage of the standard plate count?

Figure 6.2 -Figure 6.2 shows a typical bacterial growth curve with the x- axis indicating the log of the number of bacteria and the y- axis indicating time in culture. In the figure, which section (or sections) shows a growth phase where the number of cells dying equals the number of cells dividing?

Agar is used as a solidifying agent in microbiological media since few bacteria can degrade it.

Figure 6.1 -In Figure 6.1, which line best depicts a psychrotroph incubated at 0°C?