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What kind of molecules serve as electron acceptors in cellular respiration?


A) molecules with low potential energy
B) molecules with high potential energy
C) polar molecules
D) molecules in an excited state
E) water

F) A) and D)
G) A) and B)

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During cellular respiration, some bacteria use CH4 as an electron donor and O2 as an electron acceptor. What is the name for this group of bacteria?


A) nitrifiers
B) organotrophs
C) methanogens
D) methanotrophs

E) A) and B)
F) A) and C)

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You have found a prokaryote that is able to form colonies and produce oxygen. To what lineage does it belong?


A) Proteobacteria
B) Actinobacteria
C) Crenarchaeota
D) Chlamydiales
E) Cyanobacteria

F) D) and E)
G) A) and C)

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Biologists sometimes divide living organisms into two groups: autotrophs and heterotrophs. How do these two groups differ?


A) They use different sources of carbon.
B) They use different electron acceptors.
C) They use different sources of energy.
D) They differ in the way they generate ATP.

E) None of the above
F) B) and D)

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Starting in 1945, Escherichia coli became a bacterium of choice to study basic cellular processes and gene function. What specific feature of E. coli is responsible for its rise to prominence as a model system?


A) very fast growth rate
B) ability to grow in suspension culture and on agar plates
C) ability to transfer a copy of a plasmid from one cell to another
D) the fact that it has small, circular DNA

E) A) and B)
F) A) and C)

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Which of the following is correct?


A) During oxygenic photosynthesis, water is used as a source of electrons; during non- oxygenic photosynthesis, hydrogen sulfide is used as a source of electrons.
B) During oxygenic photosynthesis, hydrogen sulfide is used as a source of electrons; during non- oxygenic photosynthesis, water is used as a source of electrons.
C) During oxygenic photosynthesis, oxygen is used as a source of electrons; during non- oxygenic photosynthesis, hydrogen sulfide is used as a source of electrons.
D) During oxygenic photosynthesis, oxygen is used as a source of electrons; during non- oxygenic photosynthesis, water is used as a source of electrons.

E) A) and B)
F) All of the above

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What is the goal of bioremediation?


A) Improve bacteria for production of useful chemicals.
B) Improve soil quality for plant growth by using bacteria.
C) Improve human health with the help of living organisms such as bacteria.
D) Clean up areas polluted with toxic compounds by using bacteria.
E) All of the above are correct.

F) A) and D)
G) D) and E)

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A bacterium was grown in two test tubes filled with media rich in glucose. One tube was sealed to produce anaerobic conditions, and the other was not. A third uninoculated, unsealed tube was used as a control. Glucose utilization by this bacterium causes acid production, which is indicated by a lightening of the media colour. If the bacterium is able to produce ATP only by respiration, which result do you expect to receive?


A) lightening of media in the unsealed tube only
B) lightening of media in all three tubes
C) lightening of media in the sealed tube only
D) lightening of media in the control tube only
E) lightening of media in the sealed and unsealed tubes only

F) B) and D)
G) None of the above

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Multicellularity and large body size of eukaryotic organisms requires high metabolic rates and efficient ATP production by aerobic respiration. How did bacteria change Earth's atmosphere to enable aerobic respiration?


A) Nitrogen fixation by cyanobacteria decreased the amount of nitrogen in the atmosphere, leading to simultaneous increase of oxygen.
B) Splitting of water during anaerobic respiration by cyanobacteria dramatically increased the amount of oxygen in the atmosphere.
C) Oxygenic photosynthesis by cyanobacteria significantly increased the amount of oxygen in the atmosphere.
D) Anaerobic respiration by cyanobacteria increased the amount of oxygen in the atmosphere by reducing the amount of iron oxides that are able to react instantly with oxygen.

E) All of the above
F) B) and D)

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You might be interested to know how many different types of bacteria live on the shower curtain in your bathroom. What is the most efficient method for answering this question?


A) direct sequencing
B) enrichment culture
C) Enrichment culture and direct sequencing are equally efficient methods.

D) None of the above
E) A) and B)

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A water sample from a hot thermal vent contained a single- celled organism that had a cell wall but lacked a nucleus. What is its most likely classification?


A) Fungi
B) Animalia
C) Eukarya
D) Archaea
E) Protista

F) B) and E)
G) A) and B)

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What organisms are most numerous on Earth?


A) insects
B) plants
C) prokaryotes
D) archaea
E) eukaryotes

F) A) and E)
G) B) and E)

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When some bacteria use lactose as a source of energy, they produce propionic acid and CO2. What is the name for this process?


A) cellular respiration
B) carbon fixation
C) chemical synthesis
D) photosynthesis
E) fermentation

F) All of the above
G) B) and C)

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A biologist trying to determine the mechanism of adenosine triphosphate ATP) production in a newly discovered bacterium provides several different substrates on which the bacteria can feed. Which of the following results would indicate that the species uses fermentation to produce ATP?


A) The bacteria utilize glucose and produce carbon dioxide as a by- product of respiration.
B) The biologist discovers that the bacteria are able to produce organic molecules by metabolizing methane.
C) After exposure to amino acids, the bacteria produce a smell like rotting flesh.
D) After the bacteria break down their substrate, the biologist notices a rotten egg smell.
E) The bacteria can only produce ATP in the presence of light.

F) B) and E)
G) C) and D)

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What is the right progression of steps for a direct- sequencing experiment?


A) Lyse cells, insert genes into plasmid, purify DNA, and amplify genes by PCR.
B) Purify DNA, amplify genes by PCR, lyse cells, and insert genes into plasmid.
C) Lyse cells, insert genes into plasmid, amplify genes by PCR, and purify DNA.
D) Lyse cells, purify DNA, amplify genes by PCR, and insert genes into plasmids.

E) A) and D)
F) C) and D)

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You run an osteology lab, where students are able to study the bone structure of various species. To produce clean bones, you introduce insects and bacteria to remove all remaining flesh. Which bacteria would be the best purchase for your lab?


A) Chlamydiae
B) Spirochaetes
C) Actinobacteria
D) Proteobacteria
E) Cyanobacteria

F) D) and E)
G) A) and C)

Correct Answer

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Researchers have found a new type of bacteria, and they want to determine its phylum. What would be the most reliable method to do so?


A) Conduct a Gram- stain test.
B) Analyze the morphological characteristics of the bacteria.
C) Perform an analysis of metabolic pathways.
D) Determine the ribosomal RNA sequence of the bacteria.

E) A) and B)
F) C) and D)

Correct Answer

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You need to identify the major type of bacteria living on the shower curtain in your bathroom and find out what they use as a food source. What is the most efficient method for answering this question?


A) enrichment culture
B) direct sequencing
C) Enrichment culture and direct sequencing are equally efficient methods.

D) A) and B)
E) A) and C)

Correct Answer

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